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r2a agar medium  (Difco)

 
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    Difco r2a agar medium
    R2a Agar Medium, supplied by Difco, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/r2a+agar+medium/agar+nutrient/pmc12401914-85-22-25
    Average 86 stars, based on 1 article reviews
    r2a agar medium - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Isolation:

    Article Title: Hymenobacter properus sp. nov., Hymenobacter ruricola sp. nov., and Hymenobacter jeongseonensis sp. nov., three new species isolated from mountain and beach soil in South Korea.
    Article Snippet: Three Gram-stain-negative, aerobic, circular, convex, red-colored and rod-shaped bacterial strains, designated BT439, BT662 and BT683 were obtained from soil collected in South Korea.. The phylogenetic analysis based on 16S rRNA gene sequences of the strains revealed a distinct lineage within the genus Hymenobacter.. The complete genome sequence of strains BT439, BT662 and BT683 is 5,542,738 bp, 5,964,541 bp, and 5,192,601 bp size, respectively.

    Article Title: Spirosoma profusum sp. nov., and Spirosoma validum sp. nov., radiation-resistant bacteria isolated from soil in South Korea.
    Article Snippet: Two novel Gram-negative, rod-shaped bacterial strains BT702 and BT704 were isolated from soil collected in Jeongseon (37 220 4500 N, 128 390 5300 E), Gangwon province, South Korea.. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains BT702 and BT704 belong to distinct lineage within the genus Spirosoma (family Cytophagaceae, order Cytophagales, class Cytophagia and phylum Bacteroidetes).. The strain BT702 was closely related to Spirosoma flavus 15J11-2 (96.7% 16S rRNA gene similarity) and Spirosoma metallilatum TX0405 (93.3%).

    Incubation:

    Article Title: Hymenobacter properus sp. nov., Hymenobacter ruricola sp. nov., and Hymenobacter jeongseonensis sp. nov., three new species isolated from mountain and beach soil in South Korea.
    Article Snippet: Three Gram-stain-negative, aerobic, circular, convex, red-colored and rod-shaped bacterial strains, designated BT439, BT662 and BT683 were obtained from soil collected in South Korea.. The phylogenetic analysis based on 16S rRNA gene sequences of the strains revealed a distinct lineage within the genus Hymenobacter.. The complete genome sequence of strains BT439, BT662 and BT683 is 5,542,738 bp, 5,964,541 bp, and 5,192,601 bp size, respectively.

    Article Title: Spirosoma profusum sp. nov., and Spirosoma validum sp. nov., radiation-resistant bacteria isolated from soil in South Korea.
    Article Snippet: Two novel Gram-negative, rod-shaped bacterial strains BT702 and BT704 were isolated from soil collected in Jeongseon (37 220 4500 N, 128 390 5300 E), Gangwon province, South Korea.. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains BT702 and BT704 belong to distinct lineage within the genus Spirosoma (family Cytophagaceae, order Cytophagales, class Cytophagia and phylum Bacteroidetes).. The strain BT702 was closely related to Spirosoma flavus 15J11-2 (96.7% 16S rRNA gene similarity) and Spirosoma metallilatum TX0405 (93.3%).

    Article Title: Plant growth-promoting bacteria as biological control agents for sustainable agriculture: targeting root-knot nematodes
    Article Snippet: .. Briefly, 5 g of soil were resuspended in 50 ml of sterile NaCl 0.85% (w/v), serial dilutions were obtained and plated in R2A agar medium (Difco Laboratories, Detroit, Michigan, USA), phosphate agar medium, and zinc agar medium, and incubated at 25°C for 5 days, according to , . ..

    Article Title: Hymenobacter telluris sp. nov., isolated from soil in South Korea.
    Article Snippet: Two novel bacterial strains, designated as BT186T and BT505, were isolated from a soil sample collected in South Korea and characterized.. Both strains were Gram-stain-negative, rod-shaped, aerobic, circular, convex, and had red-colored colonies.. The level of 16S rRNA gene sequence similarity between the strains BT186T and BT505 was 100%, indicating that they represent an identical species.

    Purification:

    Article Title: Hymenobacter properus sp. nov., Hymenobacter ruricola sp. nov., and Hymenobacter jeongseonensis sp. nov., three new species isolated from mountain and beach soil in South Korea.
    Article Snippet: Three Gram-stain-negative, aerobic, circular, convex, red-colored and rod-shaped bacterial strains, designated BT439, BT662 and BT683 were obtained from soil collected in South Korea.. The phylogenetic analysis based on 16S rRNA gene sequences of the strains revealed a distinct lineage within the genus Hymenobacter.. The complete genome sequence of strains BT439, BT662 and BT683 is 5,542,738 bp, 5,964,541 bp, and 5,192,601 bp size, respectively.

    Serial Dilution:

    Article Title: Flavobacterium difficile sp. nov., isolated from a freshwater waterfall.
    Article Snippet: .. The water sample was plated on R2A agar medium (BD Difco) through serial dilution technique. ..

    Sterility:

    Article Title: Plant growth-promoting bacteria as biological control agents for sustainable agriculture: targeting root-knot nematodes
    Article Snippet: .. Briefly, 5 g of soil were resuspended in 50 ml of sterile NaCl 0.85% (w/v), serial dilutions were obtained and plated in R2A agar medium (Difco Laboratories, Detroit, Michigan, USA), phosphate agar medium, and zinc agar medium, and incubated at 25°C for 5 days, according to , . ..

    Suspension:

    Article Title: Methyloraptor flagellatus gen. nov., sp. nov., novel Ancalomicrobiaceae-affiliated facultatively methylotrophic bacteria that feed on methanotrophs of the genus Methylococcus.
    Article Snippet: A morphologically conspicuous microbial association was detected in a bioreactor running in a continuous mode with methanotrophic bacteria of the genus Methylococcus and natural gas as a growth substrate.. The association consisted of spherical Methylococcus cells colonized by elongated rods, which produced rosette-like aggregates and inhibited the cultivation process.. An isolate of these bacteria, strain S20T, was obtained and identified as belonging to the alphaproteobacterial family Ancalomicrobiaceae but displaying only a distant relationship (93.9–95.1 % 16S rRNA gene sequence similarity) to characterized members of this family.



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    Persistence of bacterial subpopulations in microcosms from the Alb river at two incubation temperatures (20 °C and 4 °C) and the Rhône river at 20 °C, with or without treated wastewater addition over 10 weeks. A abundance of total E. coli cells quantified using the IDEXX Colilert test ( N = 144). B Colony-forming units (CFU) of ESBL-producing E. coli grown on CHROMagar medium ( N = 140). C CFU of total ( N = 96) and D ESBL-producing ( N = 96) bacteria grown on <t>R2A</t> agar. RW, river water. RW + WW, river water with 5% v/v wastewater addition. Values under the detection limit (1 cell/1 CFU) for graphs A – D and missing values from the Rhône for graphs C and D are not represented
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    r2a agar medium  (Difco)
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    Persistence of bacterial subpopulations in microcosms from the Alb river at two incubation temperatures (20 °C and 4 °C) and the Rhône river at 20 °C, with or without treated wastewater addition over 10 weeks. A abundance of total E. coli cells quantified using the IDEXX Colilert test ( N = 144). B Colony-forming units (CFU) of ESBL-producing E. coli grown on CHROMagar medium ( N = 140). C CFU of total ( N = 96) and D ESBL-producing ( N = 96) bacteria grown on <t>R2A</t> agar. RW, river water. RW + WW, river water with 5% v/v wastewater addition. Values under the detection limit (1 cell/1 CFU) for graphs A – D and missing values from the Rhône for graphs C and D are not represented
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    Persistence of bacterial subpopulations in microcosms from the Alb river at two incubation temperatures (20 °C and 4 °C) and the Rhône river at 20 °C, with or without treated wastewater addition over 10 weeks. A abundance of total E. coli cells quantified using the IDEXX Colilert test ( N = 144). B Colony-forming units (CFU) of ESBL-producing E. coli grown on CHROMagar medium ( N = 140). C CFU of total ( N = 96) and D ESBL-producing ( N = 96) bacteria grown on <t>R2A</t> agar. RW, river water. RW + WW, river water with 5% v/v wastewater addition. Values under the detection limit (1 cell/1 CFU) for graphs A – D and missing values from the Rhône for graphs C and D are not represented
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    Image Search Results


    Persistence of bacterial subpopulations in microcosms from the Alb river at two incubation temperatures (20 °C and 4 °C) and the Rhône river at 20 °C, with or without treated wastewater addition over 10 weeks. A abundance of total E. coli cells quantified using the IDEXX Colilert test ( N = 144). B Colony-forming units (CFU) of ESBL-producing E. coli grown on CHROMagar medium ( N = 140). C CFU of total ( N = 96) and D ESBL-producing ( N = 96) bacteria grown on R2A agar. RW, river water. RW + WW, river water with 5% v/v wastewater addition. Values under the detection limit (1 cell/1 CFU) for graphs A – D and missing values from the Rhône for graphs C and D are not represented

    Journal: Environmental Monitoring and Assessment

    Article Title: Persistence of environmental and wastewater-associated antibiotic resistance in river water

    doi: 10.1007/s10661-026-15246-9

    Figure Lengend Snippet: Persistence of bacterial subpopulations in microcosms from the Alb river at two incubation temperatures (20 °C and 4 °C) and the Rhône river at 20 °C, with or without treated wastewater addition over 10 weeks. A abundance of total E. coli cells quantified using the IDEXX Colilert test ( N = 144). B Colony-forming units (CFU) of ESBL-producing E. coli grown on CHROMagar medium ( N = 140). C CFU of total ( N = 96) and D ESBL-producing ( N = 96) bacteria grown on R2A agar. RW, river water. RW + WW, river water with 5% v/v wastewater addition. Values under the detection limit (1 cell/1 CFU) for graphs A – D and missing values from the Rhône for graphs C and D are not represented

    Article Snippet: River water bacteria from 100 mL of Alb river microcosms incubated at room temperature and at 4 °C at every sampling time filtered using 0.45-μm pore-size filters were cultured on R2A agar medium (Merck) for 48 h at 21 °C.

    Techniques: Incubation, Bacteria